Description
Ethylenediaminetetraacetic acid, 500g
ACS reagent, 99.4-100.6%, powder
Synonym(s):
(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid
About This Item
Linear Formula:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS Number:
60-00-4
Molecular Weight:
292.24
Beilstein/REAXYS Number:
1716295
EC Number:
200-449-4
MDL number:
MFCD00003541
UNSPSC Code:
12352106
eCl@ss:
39030907
PubChem Substance ID:
24894705
NACRES:
NA.77
Properties
Quality Level
200
grade
ACS reagent
agency
suitable for SM 4500 - NH3
assay
99.4-100.6%
form
powder
reaction suitability
reagent type: chelator
impurities
≤0.005% insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)
ign. residue
≤0.2%
pH
2.5 (23 °C, 10 g/L)
mp
250 °C (dec.) (lit.)
solubility
water: soluble 0.4 g/L at 20 °C
cation traces
Ca: ≤0.001%
Fe: ≤0.005%
Mg: ≤5 ppm
heavy metals: ≤0.001% (byICP-OES)
SMILES string
OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
InChI key
KCXVZYZYPLLWCC-UHFFFAOYSA-N
Description
General description
Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.[1]
Application
Ethylenediaminetetraacetic acid has been used:
- as a component of labeling buffer, which is used in the washing step during cell fractionation{155)
- for investigating the effect of papain enzyme on wound debridement and other skin issues[2]
- for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis[3]
Biochem/physiol Actions
Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany.[4] It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.[5]
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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Safety Information
signalword
Warning
hcodes
H319
pcodes
P264 - P280 - P305 + P351 + P338 - P337 + P313
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves