Description
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
67-66-3
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
200-663-8
MDL number:
MFCD00000826
PubChem Substance ID:
24892970
grade
PCR Reagent
Quality Level
200
vapor density
4.1 (vs air)
vapor pressure
160 mmHg ( 20 °C)
assay
≥99%
form
liquid
contains
amylenes as stabilizer
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
color
colorless
refractive index
n20/D 1.445 (lit.)
bp
60.5-61.5 °C (lit.)
mp
−63 °C (lit.)
density
1.492 g/mL at 25 °C (lit.)
foreign activity
DNase, RNase, none detected
SMILES string
ClC(Cl)Cl
InChI
1S/CHCl3/c2-1(3)4/h1H
InChI key
HEDRZPFGACZZDS-UHFFFAOYSA-N
Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.
Application
Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification.
Packaging
5 vials in serum bottle
SAFETY INFORMATION
Signal Word
Danger
Hazard Statements
H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 - H412
Precautionary Statements
P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves