Description
Bromothymol Blue sodium salt solution
0.04 wt. % in H2O
Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
34722-90-2
Molecular Weight:
646.36
MDL number:
MFCD00077263
PubChem Substance ID:
24859144
NACRES:
NA.47
PROPERTIES
form
liquid
Quality Level
200
concentration
0.04 wt. % in H2O
pH
6.0-7.6, yellow to blue
density
0.99 g/mL at 25 °C (lit.)
λmax
615 nm
ε (extinction coefficient)
≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
InChI
1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
InChI key
NMKFVGALBGZKGW-FKWCIMQXSA-M
General description
Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.
Application
Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.
Biochem/physiol Actions
Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.[1]
SAFETY INFORMATION
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
-
MilliporeSigmaBromothymol blue, 5G
SKU: 1030260005
$143.00Bromothymol blue indicator ACS,Reag. Ph Eur Synonym(s): Bromothymol blue, 3′,3′′-Dibromothymolsulfonphthalein, BTB Empirical Formula (Hill Notation): C27H28Br2O5S CAS Number:</ -
MilliporeSigmaBromothymol Blue sodium salt for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6) (1g)
SKU: 18470-1G
$30.0018470 Sigma-Aldrich Bromothymol Blue sodium salt for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6) Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt, Bromthymol Blue sodium... -
MilliporeSigmaBromothymol Blue sodium salt for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6) (10g)
SKU: 18470-10G
$61.2018470 Sigma-Aldrich Bromothymol Blue sodium salt for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6) Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt, Bromthymol Blue sodium... -
MilliporeSigmaBromothymol Blue sodium salt for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6) (50g)
SKU: 18470-50G
$229.0018470 Sigma-Aldrich Bromothymol Blue sodium salt for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6) Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt, Bromthymol Blue sodium... -
MilliporeSigmaSodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O, 100mL
SKU: 72068-100ML
$122.00Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O, 100mL Linear Formula: NaOH CAS Number: 1310-73-2 Molecular Weight: 40.00 MDL number: MFCD00003548 PubChem...
