MilliporeSigma
Ascentis Express Phenyl-Hexyl, 2.7um HPLC Column, L x I.D. 10 cm x 4.6 mm, 1EA
- SKU:
- 53352-U
On Sale
Description
Ascentis® Express 90 Å Phenyl-Hexyl (2.7 μm) HPLC Columns
L × I.D. 10 cm × 4.6 mm, HPLC Column
Synonym(s):
Core-shell (SPP) Fused Core Phenyl HPLC column
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Properties
product name
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 4.6 mm
material
stainless steel column
Quality Level
100
agency
suitable for USP L11
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
10 cm × 4.6 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
phenylhexyl phase
particle size
2.7 μm
pore size
90 Å
pH range
2-9
application(s)
food and beverages
separation technique
reversed phase
Description
General description
The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.
Application
- A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers: Demonstrates the robustness of the Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC column in automating the detection of contaminants in beverages. This study showcases the column′s capacity for high throughput analysis, essential for quality control in the food industry, ensuring safety and compliance with regulatory standards (Lhotská et al., 2016).
- Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids: This research highlights the efficiency of the Ascentis® Express Phenyl-Hexyl column in analyzing complex phenolic compounds, illustrating its superior performance in environmental and pharmaceutical applications. The column′s design enables enhanced separation capabilities, crucial for accurate phenolic profiling in various samples (Chocholouš et al., 2013).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Safety Information
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable