product name

Ascentis^® Express F5, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L43

product line

Ascentis^®

feature

endcapped

manufacturer/tradename

Ascentis^®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

90 m²/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

5 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

Ascentis^® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core^® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

• Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employed the Ascentis^® Express F5, 5 µm HPLC column to determine lumacaftor and its degradation products in the presence of ivacaftor. The application of high-performance liquid chromatography ion trap time-of-flight mass spectrometry provided high sensitivity and resolution, showcasing the column′s efficacy in complex sample matrices (Özcan et al., 2023).

• Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This research highlights the use of the Ascentis^® Express F5, 5 µm HPLC column in developing stability-indicating methods for tasimelteon. The column′s performance in both LC-MS/MS and LC-DAD methods ensured accurate and reliable identification of degradation products (Özcan et al., 2020).

• Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in HPLC technology, including the Ascentis^® Express F5, 5 µm HPLC column. The article emphasizes the column′s capability in ultra-fast separations, providing enhanced efficiency and resolution in various analytical applications (Ali et al., 2012).

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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.