Ascentis Express F5, 2um UHPLC Column, L x I.D. 5 cm x 3 mm1, 1EA

SKU:
50871-U
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    • Description

    Ascentis® Express 90 Å F5 (2 μm) HPLC Columns

    L × I.D. 5 cm × 3 mm UHPLC Column

    About This Item

    UNSPSC Code:
    41115700
    NACRES:
    SB.52

    Properties

    product name

    Ascentis® Express F5, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 5 cm × 3 mm

    material

    stainless steel column

    Quality Level

    100

    agency

    suitable for USP L43

    product line

    Ascentis®

    feature

    endcapped

    manufacturer/tradename

    Ascentis®

    packaging

    1 ea of

    parameter

    1000 bar max. pressure (14500 psi)
    60 °C temp. range

    technique(s)

    LC/MS: suitable
    UHPLC-MS: suitable
    UHPLC: suitable

    L × I.D.

    5 cm × 3 mm

    surface area

    120 m2/g

    impurities

    <5 ppm metals

    matrix

    Fused-Core particle platform
    superficially porous particle

    matrix active group

    PFP (pentafluorophenyl) phase

    particle size

    2 μm

    pore size

    90 Å pore size

    operating pH

    2-8

    application(s)

    food and beverages

    separation technique

    reversed phase

    General description

    Ascentis® Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core® particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis® Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Having polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

    Application

    UHPLC/MS Analysis of Mycotoxins on Ascentis® Express F5, 2 μm

    Legal Information

    Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
    Fused-Core is a registered trademark of Advanced Materials Technology, Inc.