apHera NH2 (5 um) HPLC Column, L x I.D. 15 cm x 4.6 mm

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56401AST
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    • Description

    apHera™ NHâ‚‚ (5 μm) HPLC Columns

    L × I.D. 15 cm × 4.6 mm, HPLC Column

    Synonym(s):

    Amino HPLC Column

    About This Item

    UNSPSC Code:
    41115700
    eCl@ss:
    32110501
    NACRES:
    SB.52

    Properties

    product name

    apHera™ NH2 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

    material

    stainless steel column

    Quality Level

    100

    agency

    suitable for USP L82

    product line

    apHera™

    packaging

    pkg of 1 ea

    manufacturer/tradename

    apHera™

    parameter

    147 bar pressure (2133 psi)
    4-50 °C temperature

    technique(s)

    HPLC: suitable
    LC/MS: suitable

    L × I.D.

    15 cm × 4.6 mm

    matrix

    polymeric

    matrix active group

    amino phase

    particle size

    5 μm

    pore size

    300 Å

    operating pH

    1-14

    application(s)

    food and beverages

    separation technique

    hydrophilic interaction (HILIC)
    normal phase

    General description

    apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

    Features of Amino apHera™ Columns
    • High efficiency for carbohydrate analysis
    • Ideal for basic conditions/amphoteric detection
    • High selectivity mono- to oligosaccharides
    • pH range 2-13
    • 300 angstrom pore size
    • Stable, predictable retention
    • Long column life

    Legal Information

    apHera is a trademark of Sigma-Aldrich Co. LLC