apHera C4 HPLC Columns, 15 cm x 4.6 mm (5 um particles)

SKU:
56302AST
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    • Description

    apHera™ C4 (5 μm) HPLC Columns

    L × I.D. 15 cm × 4.6 mm, HPLC Column

    Synonym(s):

    Polymeric C4 Reversed-Phase Column

    About This Item

    UNSPSC Code:
    41115700
    eCl@ss:
    32110501
    NACRES:
    SB.52

    Properties

    product name

    apHera™ C4 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

    material

    stainless steel column

    Quality Level

    100

    product line

    apHera™

    packaging

    pkg of 1 ea

    manufacturer/tradename

    apHera™

    parameter

    147 bar pressure (2133 psi)
    4-60 °C temperature

    technique(s)

    HPLC: suitable
    LC/MS: suitable

    L × I.D.

    15 cm × 4.6 mm

    matrix

    polymeric

    matrix active group

    C4 (butyl) phase

    particle size

    5 μm

    pore size

    300 Å

    operating pH

    1-14

    application(s)

    food and beverages

    separation technique

    reversed phase

    General description

    apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either. This was accomplished using a vinyl alcohol copolymer base that keeps the surface wetted even with high carbon loads. The porous structure has an average pore diameter large enough to produce ideal results for small analytes, peptides and small proteins. These column equal silica based columns in separation efficiency with organic solvents but provide efficiency with buffered alkaline solutions not possible on silica. One of the most significant features in logicial elution order of alkylated bases where retention increases proportionately with increasing chain length.

    Features of apHera™ Reversed Phase Columns
    • Stable vinyl copolymer base
    • Stable pH range 2-12
    • 300 angstrom pore size
    • Stable in all organic solvents
    • Expanded applications: peptides, proteins
    • Efficient separation of any basic substance
    • Amenability to washing and alkaline solutions

    Legal Information

    apHera is a trademark of Sigma-Aldrich Co. LLC