Anti-Cannabinoid Receptor 1 Antibody, CT Chemicon, from rabbit

SKU:
AB5638P
  • $526.00

    • Description

    Anti-Cannabinoid Receptor 1 Antibody, CT Chemicon, from rabbit

    Synonym(s):
    CB1
    eCl@ss:
    32160702
    NACRES:
    NA.41

    biological source

    rabbit

    Quality Level

    100

    antibody form

    affinity purified immunoglobulin

    antibody product type

    primary antibodies

    clone

    polyclonal

    purified by

    affinity chromatography

    species reactivity

    mouse, rat

    manufacturer/tradename

    Chemicon®

    application(s)

    ELISA: suitable
    western blot: suitable

    NCBI accession no.

    NM_016083.3
    NM_033181.2

    UniProt accession no.

    P21554

    shipped in

    dry ice

    CB1 is a protein that is one of two cannabinoid receptors (CB1 CB2). Cannabinoid principally delta-9-tetrahydrocannabinol (THC) and its synthetic analogs, are the active psychoactive compounds present is marijuana. These cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptors which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Three splice variants have been found for this gene. CB1 is primarily located in brain, and CB2 is found macrophages.

    Specificity

    Recognizes rat Cannabinoid Receptor 1 (CB1). The immunogen shows no significant sequence homology with CB2 or other receptors.

    SPECIES REACTIVITIES: The immunogen peptide is 100% conserved mouse, 94% in monkey and human, 88% in zebra finch, 75% in feline and newt, (salamander) and 73% in puffer fish. Reactivity with other species has not been confirmed.

    Immunogen

    Epitope: C-terminus
    A 20 amino acid peptide sequence near the cytoplasmic, C-terminus of rat CB1 (Gerard et al. 1991; Gerard et al.

    1990; Shire et al. 1995; Matsuda et al. 1990; Ho et al. 1996; Chakraborty et al. 1995).

    Application

    Anti-Cannabinoid Receptor 1 Antibody, C-terminus is an antibody against Cannabinoid Receptor 1 for use in ELISA & WB.
    Research Category
    Neuroscience
    Western blot: 1-10 μg/mL using ECL. Expected sizes are ~60kDa and 80-84kDa under reducing conditions.

    Immunohistochemistry: not tested. It is recommended that the antibody be tried at 2-10 μg/mL on paraformaldehyde fixed tissue.

    ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog number AG213) per well.

    Optimal working dilutions must be determined by the end user.
    Research Sub Category
    Neuroinflammation & Pain

    Neurotransmitters & Receptors

    Physical form

    Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.

    Storage and Stability

    Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

    Other Notes

    Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

    Legal Information

    CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

    Disclaimer

    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

    SAFETY INFORMATION

    WGK Germany

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable